overlap-PCR

基本解释

The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.
采用重叠PCR技术 ,以抗乙肝表面抗原 (HBsAg)IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。
Methods: The gene fragment encoding the Z domain of Protein A(Protein A-Z) was generated by Overlap PCR amplification, and was cloned into TA-cloning vector pMD-18T.
方法:应用Overlap PCR的方法制备Protein A的Z结构域(Protein A-Z),克隆于pMD-18T载体构建PA-Z/pMD-18T重组质粒。
By designing four primers for an overlapping PCR, we created a fusion gene Ddl cat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase(CAT).
通过设计4个引物进行重叠（overlapping）PCR，由此克隆了人肿瘤坏死因子受体I死亡域（deathdomain）与氯霉素乙酰转移酶（CAT）的融合蛋白基因（DdLcat）。
Keywords Lagurus lagurus;zona pellucida 3;overlap PCR;codon optimization;prokaryotic expression;
草原兔尾鼠;卵透明带3;重叠PCR;密码子优化;原核表达;
There is no question of overlap between the two courses.
这两门课程之间不存在重叠的问题。