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With many genomes, coden bias provides a useful means of assigning a degree of certainty to a possible gene identification. 至于许多基因组,基码偏差为可能基因鉴定分配确定度提供有用的方法。
Methods:The full length L1 coden region of HPV58 was amplified by PCR,and cloned into pUC19,sequenced. 方法 :用聚合酶链反应 (PCR)扩增HPV58L1完整编码区基因 ,将PCR扩增产物克隆至 pUC1 9质粒中并测序。
Among the new genes (alleles), CYP4C23 may be a pseudogene, CYP4H13 has a retained intron 58 nucleotides in length, and CYP4J4V1 has a stop coden (TAG) in frame near the 3'-end. 其中CYP4C23可能是一 个假基因,CYP4H13具有一段58个碱基长度的内含子,CYP4J4V1在近3’端具有一个终止密码子TAG.
Keywords Hepatitis C virus;3 untranslated region;Stop coden; 关键词肝炎病毒;丙型;非编码区;3’端;终止密码子;
Analysing the Coden and the Conservative Character of AUG Context in Aeropyrum pernix 敏捷气热菌密码子及AUG侧翼序列保守性分析
Cloning of Human Lysozyme Coden Gene and Expressing in Pichia Pastoris for Natural Food Antiseptic 天然食品防腐剂人溶菌酶编码基因的克隆及其在毕赤酵母中的表达