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The recombinants with the cDNA insert fragment were released from the positive clones and were digested with EcoR I , The cDNA fragment were 2.1kb and 1.5kb in lycB and IPI positive clones respectively. 释放质粒后，进行酶切鉴定，LycB阳性克隆cDNA插入片段的大小为2.;1kb左右，IPI阳性克降cDNA插入片段的大小约为1